Regulatory

Part:BBa_K625002:Design

Designed by: Michael Eichenberger   Group: iGEM11_ETH_Zurich   (2011-09-21)


Pu promoter long version with stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole promoter region with all putative regulatory sequences was BioBricked. Stop codons were added in frame with the XylU coding region.


Source

PCR reaction on plasmid DNA provided by Prof. Sven Panke. pCK04AxylR

References